Journal: Acta Neuropathologica Communications
Article Title: SPION + macrophage traffic out of the central nervous system with SIV infection
doi: 10.1186/s40478-025-02179-6
Figure Lengend Snippet: SPION localize to CNS macrophage. SPION (visible as amber beads) were detected within Iba1 + , CD68 + , CD163 + , and CD206 + perivascular, meningeal, and choroid plexus macrophages in the CNS. Macrophages were identified by hematoxylin and Eosin (H&E) staining and by immunohistochemistry (IHC) for Iba1, CD68, CD163, and CD206. Rows depict representative examples of perivascular, meningeal, and choroid plexus macrophages identified by anatomical location and IHC labeling. SPION are indicated by arrowheads in magnified images. Data are representative of N = 2 non-infected monkeys, 14 days post i.c. SPION inoculation
Article Snippet: IHC was performed as previously described, using antibodies targeting CD163 (1:250, Leica (Deer Park, IL)), CD68 (1:100, DAKO (Carpinteria, CA)), CD206 (1:1000, R&D Systems (Minneapolis, MN)), and IBA1 macrophages (1:100, Wako (Osaka, Japan)) [ , ].
Techniques: Staining, Immunohistochemistry, Labeling, Infection